counterstaining with hematoxylin Search Results


t syk brown  (Bio-Techne corporation)
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hematoxylin  (Boster Bio)
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counterstain hematoxylin  (Beyotime)
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hematoxylin counterstain  (Biomeda corporation)
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gill’s hematoxylin i:dh2o counterstain  (American MasterTech Scientific Inc)
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hematoxylin counterstain  (Fisher Scientific)
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hematoxylin counterstain  (Biocare Medical)
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Biocare Medical hematoxylin counterstain
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hematoxylin counterstaining  (ZSGB Biotech)
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ZSGB Biotech hematoxylin counterstaining
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hematoxylin dab  (ZSGB Biotech)
90
ZSGB Biotech hematoxylin dab
The biological functions of AATBC. (A) Would healing assays were monitored at 0, 12, and 24 h in AATBC knocked down or overexpressed NPC cells. The gap distance was showed as mean ± SEM of three independent experiments. Statistical significance is evaluated by t ‐test. ** P < 0.01; *** P < 0.001. (B) AATBC promoted invasiveness in 5‐8F spheroid clones and the formation of filopodia in 3D cell culture model. The black arrow indicates protrusions formed on the surface of the spheroid (top), scale bars = 50 μm. The graphs represent the quantification of invasive and noninvasive spheroid types in siAATBC and the AATBC‐overexpressing cells. (C) Transwell Matrigel assays were performed in NPC cells transfected with siAATBC and the AATBC overexpression vector with their respective empty vector controls. The relative proportion of invading cells in each field is shown as mean ± SEM of three independent tests. Statistical significance is evaluated by t ‐test, ** P < 0.05; ** P < 0.01. (D) Bright‐field images of metastatic nodules in the lung tissues of mice, n = 6. (E) Significant metastases were visible in the lungs of all the three groups of mice, and arrows indicate colonies of lung tumor cells. (F) Graph representing the number of metastatic nodules observed in nude mice. Data presented as mean ± SEM (each data point represents a nude mouse, n = 6). ** P < 0.01; *** P < 0.001. (G) The microscopic images of mouse lung biopsies stained by <t>hematoxylin</t> and eosin; the rectangular box represents the clusters of micrometastatic cells in the mouse lung. The pictures were captured at 100× (scale bars = 100 μm), 200× (scale bars = 50 μm), and 400× (scale bars = 20 μm) magnifications.
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lillie-mayer hematoxylin counterstain  (Biocare Medical)
90
Biocare Medical lillie-mayer hematoxylin counterstain
The biological functions of AATBC. (A) Would healing assays were monitored at 0, 12, and 24 h in AATBC knocked down or overexpressed NPC cells. The gap distance was showed as mean ± SEM of three independent experiments. Statistical significance is evaluated by t ‐test. ** P < 0.01; *** P < 0.001. (B) AATBC promoted invasiveness in 5‐8F spheroid clones and the formation of filopodia in 3D cell culture model. The black arrow indicates protrusions formed on the surface of the spheroid (top), scale bars = 50 μm. The graphs represent the quantification of invasive and noninvasive spheroid types in siAATBC and the AATBC‐overexpressing cells. (C) Transwell Matrigel assays were performed in NPC cells transfected with siAATBC and the AATBC overexpression vector with their respective empty vector controls. The relative proportion of invading cells in each field is shown as mean ± SEM of three independent tests. Statistical significance is evaluated by t ‐test, ** P < 0.05; ** P < 0.01. (D) Bright‐field images of metastatic nodules in the lung tissues of mice, n = 6. (E) Significant metastases were visible in the lungs of all the three groups of mice, and arrows indicate colonies of lung tumor cells. (F) Graph representing the number of metastatic nodules observed in nude mice. Data presented as mean ± SEM (each data point represents a nude mouse, n = 6). ** P < 0.01; *** P < 0.001. (G) The microscopic images of mouse lung biopsies stained by <t>hematoxylin</t> and eosin; the rectangular box represents the clusters of micrometastatic cells in the mouse lung. The pictures were captured at 100× (scale bars = 100 μm), 200× (scale bars = 50 μm), and 400× (scale bars = 20 μm) magnifications.
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hematoxylin counterstaining  (Merck KGaA)
90
Merck KGaA hematoxylin counterstaining
The biological functions of AATBC. (A) Would healing assays were monitored at 0, 12, and 24 h in AATBC knocked down or overexpressed NPC cells. The gap distance was showed as mean ± SEM of three independent experiments. Statistical significance is evaluated by t ‐test. ** P < 0.01; *** P < 0.001. (B) AATBC promoted invasiveness in 5‐8F spheroid clones and the formation of filopodia in 3D cell culture model. The black arrow indicates protrusions formed on the surface of the spheroid (top), scale bars = 50 μm. The graphs represent the quantification of invasive and noninvasive spheroid types in siAATBC and the AATBC‐overexpressing cells. (C) Transwell Matrigel assays were performed in NPC cells transfected with siAATBC and the AATBC overexpression vector with their respective empty vector controls. The relative proportion of invading cells in each field is shown as mean ± SEM of three independent tests. Statistical significance is evaluated by t ‐test, ** P < 0.05; ** P < 0.01. (D) Bright‐field images of metastatic nodules in the lung tissues of mice, n = 6. (E) Significant metastases were visible in the lungs of all the three groups of mice, and arrows indicate colonies of lung tumor cells. (F) Graph representing the number of metastatic nodules observed in nude mice. Data presented as mean ± SEM (each data point represents a nude mouse, n = 6). ** P < 0.01; *** P < 0.001. (G) The microscopic images of mouse lung biopsies stained by <t>hematoxylin</t> and eosin; the rectangular box represents the clusters of micrometastatic cells in the mouse lung. The pictures were captured at 100× (scale bars = 100 μm), 200× (scale bars = 50 μm), and 400× (scale bars = 20 μm) magnifications.
Hematoxylin Counterstaining, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hematoxyline  (Merck & Co)
90
Merck & Co hematoxyline
The biological functions of AATBC. (A) Would healing assays were monitored at 0, 12, and 24 h in AATBC knocked down or overexpressed NPC cells. The gap distance was showed as mean ± SEM of three independent experiments. Statistical significance is evaluated by t ‐test. ** P < 0.01; *** P < 0.001. (B) AATBC promoted invasiveness in 5‐8F spheroid clones and the formation of filopodia in 3D cell culture model. The black arrow indicates protrusions formed on the surface of the spheroid (top), scale bars = 50 μm. The graphs represent the quantification of invasive and noninvasive spheroid types in siAATBC and the AATBC‐overexpressing cells. (C) Transwell Matrigel assays were performed in NPC cells transfected with siAATBC and the AATBC overexpression vector with their respective empty vector controls. The relative proportion of invading cells in each field is shown as mean ± SEM of three independent tests. Statistical significance is evaluated by t ‐test, ** P < 0.05; ** P < 0.01. (D) Bright‐field images of metastatic nodules in the lung tissues of mice, n = 6. (E) Significant metastases were visible in the lungs of all the three groups of mice, and arrows indicate colonies of lung tumor cells. (F) Graph representing the number of metastatic nodules observed in nude mice. Data presented as mean ± SEM (each data point represents a nude mouse, n = 6). ** P < 0.01; *** P < 0.001. (G) The microscopic images of mouse lung biopsies stained by <t>hematoxylin</t> and eosin; the rectangular box represents the clusters of micrometastatic cells in the mouse lung. The pictures were captured at 100× (scale bars = 100 μm), 200× (scale bars = 50 μm), and 400× (scale bars = 20 μm) magnifications.
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Image Search Results


The biological functions of AATBC. (A) Would healing assays were monitored at 0, 12, and 24 h in AATBC knocked down or overexpressed NPC cells. The gap distance was showed as mean ± SEM of three independent experiments. Statistical significance is evaluated by t ‐test. ** P < 0.01; *** P < 0.001. (B) AATBC promoted invasiveness in 5‐8F spheroid clones and the formation of filopodia in 3D cell culture model. The black arrow indicates protrusions formed on the surface of the spheroid (top), scale bars = 50 μm. The graphs represent the quantification of invasive and noninvasive spheroid types in siAATBC and the AATBC‐overexpressing cells. (C) Transwell Matrigel assays were performed in NPC cells transfected with siAATBC and the AATBC overexpression vector with their respective empty vector controls. The relative proportion of invading cells in each field is shown as mean ± SEM of three independent tests. Statistical significance is evaluated by t ‐test, ** P < 0.05; ** P < 0.01. (D) Bright‐field images of metastatic nodules in the lung tissues of mice, n = 6. (E) Significant metastases were visible in the lungs of all the three groups of mice, and arrows indicate colonies of lung tumor cells. (F) Graph representing the number of metastatic nodules observed in nude mice. Data presented as mean ± SEM (each data point represents a nude mouse, n = 6). ** P < 0.01; *** P < 0.001. (G) The microscopic images of mouse lung biopsies stained by hematoxylin and eosin; the rectangular box represents the clusters of micrometastatic cells in the mouse lung. The pictures were captured at 100× (scale bars = 100 μm), 200× (scale bars = 50 μm), and 400× (scale bars = 20 μm) magnifications.

Journal: Molecular Oncology

Article Title: LncRNA AATBC regulates Pinin to promote metastasis in nasopharyngeal carcinoma

doi: 10.1002/1878-0261.12703

Figure Lengend Snippet: The biological functions of AATBC. (A) Would healing assays were monitored at 0, 12, and 24 h in AATBC knocked down or overexpressed NPC cells. The gap distance was showed as mean ± SEM of three independent experiments. Statistical significance is evaluated by t ‐test. ** P < 0.01; *** P < 0.001. (B) AATBC promoted invasiveness in 5‐8F spheroid clones and the formation of filopodia in 3D cell culture model. The black arrow indicates protrusions formed on the surface of the spheroid (top), scale bars = 50 μm. The graphs represent the quantification of invasive and noninvasive spheroid types in siAATBC and the AATBC‐overexpressing cells. (C) Transwell Matrigel assays were performed in NPC cells transfected with siAATBC and the AATBC overexpression vector with their respective empty vector controls. The relative proportion of invading cells in each field is shown as mean ± SEM of three independent tests. Statistical significance is evaluated by t ‐test, ** P < 0.05; ** P < 0.01. (D) Bright‐field images of metastatic nodules in the lung tissues of mice, n = 6. (E) Significant metastases were visible in the lungs of all the three groups of mice, and arrows indicate colonies of lung tumor cells. (F) Graph representing the number of metastatic nodules observed in nude mice. Data presented as mean ± SEM (each data point represents a nude mouse, n = 6). ** P < 0.01; *** P < 0.001. (G) The microscopic images of mouse lung biopsies stained by hematoxylin and eosin; the rectangular box represents the clusters of micrometastatic cells in the mouse lung. The pictures were captured at 100× (scale bars = 100 μm), 200× (scale bars = 50 μm), and 400× (scale bars = 20 μm) magnifications.

Article Snippet: The sections were incubated with anti‐AATBC oligodeoxynucleotide probe that was conjugated with antidigoxin at 37 °C humidified chamber for 16 h. After hybridization, the sections were washed in 1× PBS for 5 min and stained with hematoxylin (DAB, ZSGB‐BIO, Beijing, China).

Techniques: Clone Assay, Cell Culture, Transfection, Over Expression, Plasmid Preparation, Staining